Other

Part:BBa_K2983023:Design

Designed by: Tom Zaplana   Group: iGEM19_Evry_Paris-Saclay   (2019-10-11)


Loop Omega-E


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 18
    Illegal SapI site found at 1


Design Notes

BsaI site that leaves a GCTT overhang

SapI site that leaves a GGT overhang


Source

DNA synthesis

References